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Pyramidal tract reconstruction in vivo

Pyramidal tract of an HCP subject reconstructed in vivo using Radial DSI and ODF-Fingerprinting. Improved reconstruction of fibers crossing at shallow angles ensured by ODF-Fingerprinting allowed to reproduce the reach fanning shape of cortical terminations of the tract. Images were rendered in DSI Studio.

Full name Patryk Filipiak

Submission Type Video

Institution or organization NYU School of Medicine

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Thinking about a Greener Future

Mouse brain showing green AAV-transduced cells in the cortex and hippocampus. Neurons are labeled red and nuclei are blue.

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Evaluating Meninges during Migraine Attack: Biobot Live Feed

This image was created with confocal microscopy, FIJI processing, Blender rendering, and Photoshop (to produce Biobot nanoship). Rat meningeal tissue was labeled with antibodies against proteins involved in chronic migraine headaches. Calcitonin gene-related peptide (CGRP, red), and CGRP receptor proteins, receptor activity modifying protein 1 (RAMP1, blue) and calcitonin-like receptor (CLR, green). Geometry of meningeal... more »

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Neuron on Fire

Hippocampal CA1 pyramidal neuron in the mouse, recorded from the distal dendrite using patch clamp electrophysiology. The recorded location is visible as the gap in the dendrite. The neuron was filled with biocytin during recording and immunostained with streptavidin-647 post hoc. Confocal image was filtered using ImageJ.

Full name Olesia Bilash

Submission Type Photo

Institution or organization New York University

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Ajrak Print on the Corticospinal Tract (CST) Left

This is Sindhi Ajrak print digitally painted on the Corticospinal Tract (CST) Left. Ajrak print is 3000 years old unique form of block-printing found in Sindh, Pakistan. Ajrak print is mostly hand-painted on shawls made using block printing by stamps. Ajrak has become a symbol of the Sindhi culture and traditions. Using my programming skills, I painted Ajrak on my favorite white matter tract, CST.

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Axonal processes in the larval zebrafish brain

3D reconstruction acquired using confocal microscopy. Dorsal habenular neurons express mCherry (magenta). Neurons producing Relaxin-3 neuropeptide express GFP (green).

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Microvessels Fairylights

Isolated cerebral microvessels from transgenic sickle mouse. Immunofluorescence staining with red (CD31, endothelial cell marker) and green (Aquaporin-4, astrocyte marker), counterstained with sparkling blue (DAPI, nuclei detector).

Submission Type Photo

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Model of mouse V1 with a Neuropixels probe

This rendering of a model of mouse primary visual cortex with a Neuropixels probe was created using a new tool, VND (Visual Neuronal Dynamics).

Full name Barry Isralewitz, John Stone, Mariano Spivak, Kael Dai, Josh Siegle, Emad Tajkhorshid, and Anton Arkhipov

Submission Type Photo

Institution or organization University of Illinois at Urbana-Champaign and Allen Institute

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Super-resolved dendrites in visual cortical circuits

3D super-resolution image of immunofluorescence-labeled ChR2–EYFP on dendrites in 50-μm-thick brain section of visual cortical circuits.

Submission Type Photo

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Corticostriatal neurons of mouse anterior cingulate cortex

These neurons were labeled with dG-RV-4eGFP (courtesy of Ian Wickersham's lab) injected into the dorsomedial caudoputamen, which retrogradely infected this population in the ACAd causing them to express green fluorescent protein. This image was captured in a sagittal section, and since these neurons have an orientation perpendicular to the sagittal plane, we are viewing them from below, which is a unique perspective for... more »

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Fabricated Rhythm

Slit scan of (top) hippocampal waves measured by multi-electrode array blow virtual flags (data: Buzsaki lab) and (bottom) wind blows real flags filmed on a San Francisco pier.

Submission Type Photo

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Neverland Vein

In a woman presenting with small centrum semiovale hemorrhage, arterial DSA with rotational angiography revealed no evidence of aneurysm or AVM. Rather, it showed a large developmental venous anomaly and, for better visualization, a venous 3D DSA was also obtained. The 2 subtracted volumes were then fused to show concomitant representation of the arteries and veins. Developmental venous anomaly is an embryologically arrested... more »

Submission Type Video

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Reconstructed Mouse Cervical Spinal Cord

Serially sectioned spinal cord with marked coordinates of V1-Interneurons and cells infected by neuronal tracer pseudorabies virus - 3D reconstructed.

Full name Jamie Anne Mortel

Submission Type Video

Institution or organization Salk Institute for Biological Studies